Human SECR in complex with an engineered Gs heterotrimer

PDB ID: 7D3S RCSB | OPM

Receptor name: Secretin receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 2.9

Effector protein: G: α β γ (A B G)

Other protein: nanobody Nb35 (N)

Peptide ligand: Secretin (P)

MD preparation

Number of mutations: 8

List of mutations: (E)G50D (E)E51N (E)A345D (E)E348D (E)M450A (E)V453I (G)C106S (G)C107S

Receptor

Name: Secretin receptor

Organism: Homo sapiens

Uniprot: P47872

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Rattus norvegicus

Uniprot: P54311

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Bos taurus

Uniprot: P63212

Title: Structure of the human secretin receptor coupled to an engineered heterotrimeric G protein.

Authors: Fukuhara, S., Kobayashi, K., Kusakizako, T., Iida, W., Kato, M., Shihoya, W., Nureki, O.

Published: Biochem.Biophys.Res.Commun. (2020)

Name: nanobody Nb35 (N)

Organism: unidentified

TM regions (from OPM database)

TM1( 141-163),TM2( 177-198),TM3( 213-238),TM4( 257-279),TM5( 292-315),TM6( 341-362),TM7( 365-391)

Additional info

Membrane Thickness: 29.8 ± 1.2 Å

Tilt: 12 ± 1°