Cryo-EM structure of the INT-777-bound GPBAR-Gs complex

PDB ID: 7CFN RCSB | OPM

Receptor name: G-protein coupled bile acid receptor 1

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.0

Effector protein: G: α β γ (A B G)

Other protein: Nanobody-35 (N)

Non-peptide ligand code: FX0 (R)

MD preparation

Number of mutations: 8

List of mutations: (A)S54N, (A)G226A, (A)E268A, (A)N271K, (A)K274D, (A)R280K, (A)T284D, (A)I285T

Receptor

Name: G-protein coupled bile acid receptor 1

Organism: Homo sapiens

Uniprot: Q8TDU6

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Structural basis of GPBAR activation and bile acid recognition.

Authors: Yang, F., Mao, C., Guo, L., Lin, J., Ming, Q., Xiao, P., Wu, X., Shen, Q., Guo, S., Shen, D.D., Lu, R., Zhang, L., Huang, S., Ping, Y., Zhang, C., Ma, C., Zhang, K., Liang, X., Shen, Y., Nan, F., Yi, F., Luca, V.C., Zhou, J., Jiang, C., Sun, J.P., Xie, X., Yu, X., Zhang, Y.

Published: Nature (2020)

Name: Nanobody-35 (N)

Organism: synthetic construct

TM regions (from OPM database)

TM1( 20-40),TM2( 51-72),TM3( 86-108),TM4( 124-144),TM5( 161-186),TM6( 226-249),TM7( 263-283)

Additional info

Membrane Thickness: 31.4 ± 2.0 Å

Tilt: 4 ± 2°