Structural Basis of the Activation of Heterotrimeric Gs-protein by Isoproterenol-bound Beta1-Adrenergic Receptor

PDB ID: 7JJO RCSB | OPM

Receptor name: Beta-1 adrenergic receptor

Receptor chain: R

Receptor organism: Meleagris gallopavo

Family / Class: Rhodopsin-like / A

Resolution [Å]: 2.6

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35 (N)

Non-peptide ligand code: 5FW (R)

MD preparation

Number of mutations: 1

List of mutations: (G)C68S

Receptor

Name: Beta-1 adrenergic receptor

Organism: Meleagris gallopavo

Uniprot: P07700

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Bos taurus

Uniprot: P04896

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Bos taurus

Uniprot: P62871

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Bos taurus

Uniprot: P63212

Title: Structural Basis of the Activation of Heterotrimeric Gs-Protein by Isoproterenol-Bound beta 1 -Adrenergic Receptor.

Authors: Su, M., Zhu, L., Zhang, Y., Paknejad, N., Dey, R., Huang, J., Lee, M.Y., Williams, D., Jordan, K.D., Eng, E.T., Ernst, O.P., Meyerson, J.R., Hite, R.K., Walz, T., Liu, W., Huang, X.Y.

Published: Mol.Cell (2020)

Name: Nanobody 35 (N)

Organism: Lama glama

TM regions (from OPM database)

TM1( 42-64),TM2( 78-103),TM3( 112-137),TM4( 158-179),TM5( 205-228),TM6( 289-315),TM7( 323-343)

Additional info

Membrane Thickness: 31.8 ± 1.4 Å

Tilt: 6 ± 1°