Class D GPCR Ste2 dimer coupled to two G proteins

PDB ID: 7AD3m RCSB | OPM

Receptor name: Pheromone alpha factor receptor

Receptor chain: A,B

Receptor organism: Saccharomyces cerevisiae

Family / Class: Pheromone / D

Resolution [Å]: 3.5

Effector protein: G: α β γ (E,H F,L G,J)

Peptide ligand: Alpha-factor mating pheromone (I,K)

Non-peptide ligand code: Y01 (A,B)

MD preparation

Number of mutations: 16

List of mutations: (E)G50D (E)E51N (E)A345D (E)E348D (E)M450A (E)V453I (G)C106S (G)C107S (H)G50D (H)E51N (H)A345D (H)E348D (H)M450A (H)V453I (J)C106S (J)C107S

G protein from 1st monomer was superimposed into C-terminal helix of Galpha of 2nd monomer. Chain letters for new G protein subunits: beta - L, gamma - J.

Receptor

Name: Pheromone alpha factor receptor

Organism: Saccharomyces cerevisiae

Uniprot: P0CI39

Name: Guanine nucleotide-binding protein alpha-1 subunit

Organism: Saccharomyces cerevisiae S288C

Uniprot: P08539

Name: STE4 isoform 1

Organism: Saccharomyces cerevisiae

Uniprot: A0A6A5Q727

Name: Guanine nucleotide-binding protein subunit gamma

Organism: Saccharomyces cerevisiae

Uniprot: A0A6A5PT44

Title: Structure of the class D GPCR Ste2 dimer coupled to two G proteins.

Authors: Velazhahan, V., Ma, N., Pandy-Szekeres, G., Kooistra, A.J., Lee, Y., Gloriam, D.E., Vaidehi, N., Tate, C.G.

Published: Nature (2021)

TM regions (from OPM database)

A: TM1(50-72), TM2(79-100), TM3(129-151), TM4(162-186), TM5(202-224), TM6(247-267), TM7(274-295), B: TM1(50-72), TM2(79-100), TM3(129-151), TM4(162-186), TM5(203-224), TM6(247-267), TM7(274-295)

Additional info

Membrane Thickness: 30.2 ± 0.8 Å

Tilt: 1 ± 0°