Structure of human SMO-G111C/I496C complex with Gi

PDB ID: 6XBK RCSB | OPM

Receptor name: Smoothened homolog

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Frizzled / F

Resolution [Å]: 3.2

Effector protein: G: α β γ (A B G)

Other protein: scFv16

MD preparation

Number of mutations: 2

List of mutations: (R)G111C (R)I496C

Receptor

Name: Smoothened homolog

Organism: Homo sapiens

Uniprot: Q99835

Name: Guanine nucleotide-binding protein G(i) subunit alpha-1

Organism: Homo sapiens

Uniprot: P63096

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Sterols in an intramolecular channel of Smoothened mediate Hedgehog signaling.

Authors: Qi X, Friedberg L, De Bose-Boyd R, Long T, Li X

Published: Nat Chem Biol (2020)

Name: scFv16

Organism: Mus musculus

TM regions (from OPM database)

TM1(232-254),TM2(265-282),TM3(313-337),TM4(359-378),TM5(398-420),TM6(450-471),TM7(517-536)

Additional info

Membrane Thickness: 29.8 ± 1.6 Å

Tilt: 10 ± 1°