CryoEM Structure of the glucagon receptor with a dual-agonist peptide

PDB ID: 6WHC RCSB | OPM

Receptor name: Glucagon receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 3.4

Effector protein: G: α β γ (A B C)

Other protein: Nanobody 35

Peptide ligand: Dual-agonist peptide 15 (E)

MD preparation

Number of mutations: 0

List of mutations: None

Receptor

Name: Glucagon receptor

Organism: Homo sapiens

Uniprot: P47871

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Cryo-electron microscopy structure of the glucagon receptor with a dual-agonist peptide.

Authors: Chang R, Zhang X, Qiao A, Dai A, Belousoff MJ, Tan Q, Shao L, Zhong L, Lin G, Liang YL, Ma L, Han S, Yang D, Danev R, Wang MW, Wootten D, Wu B, Sexton PM

Published: J Biol Chem (2020)

Name: Nanobody 35

Organism: Lama glama

TM regions (from OPM database)

TM1(144-166),TM2(174-193),TM3(228-249),TM4(264-284),TM5(304-326),TM6(380-404)

Additional info

Membrane Thickness: 28.6 ± 2 Å

Tilt: 5 ± 1°