Cryo-EM structure of the Glucagon-like peptide-1 receptor in complex with G protein, GLP-1 peptide and a positive allosteric modulator

PDB ID: 6VCB RCSB | OPM

Receptor name: Glucagon-like peptide 1 receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 3.3

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

Peptide ligand: Glucagon-like peptide 1 (P)

Non-peptide ligand code: QW7 (R)

MD preparation

Number of mutations: 0

List of mutations: None

Receptor

Name: Glucagon-like peptide 1 receptor

Organism: Homo sapiens

Uniprot: P43220

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P43220

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Structural insights into probe-dependent positive allosterism of the GLP-1 receptor.

Authors: Bueno AB, Sun B, Willard FS, Feng D, Ho JD, Wainscott DB, Showalter AD, Vieth M, Chen Q, Stutsman C, Chau B, Ficorilli J, Agejas FJ, Cumming GR, Jiménez A, Rojo I, Kobilka TS, Kobilka BK, Sloop KW

Published: Nat Chem Biol (2020)

Name: Nanobody 35

Organism: Lama glama

TM regions (from OPM database)

TM1(143-168),TM2(177-199),TM3(227-249),TM4(268-290),TM5(303-328),TM6(349-375),TM7(379-405)

Additional info

Membrane Thickness: 31 ± 1 Å

Tilt: 16 ± 1°