CryoEM Structure of the active Adrenomedullin 2 receptor G protein complex with adrenomedullin 2 peptide

PDB ID: 6UVA RCSB | OPM

Receptor name: Calcitonin gene-related peptide type 1 receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 2.3

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

Peptide ligand: Adrenomedullin peptide (P), Receptor activity-modifying protein 3 (E)

MD preparation

Number of mutations: 0

List of mutations: None

Receptor

Name: Calcitonin gene-related peptide type 1 receptor

Organism: Homo sapiens

Uniprot: Q16602

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Structure and Dynamics of Adrenomedullin Receptors AM₁ and AM₂ Reveal Key Mechanisms in the Control of Receptor Phenotype by Receptor Activity-Modifying Proteins.

Authors: Liang YL, Belousoff MJ, Fletcher MM, Zhang X, Khoshouei M, Deganutti G, Koole C, Furness SGB, Miller LJ, Hay DL, Christopoulos A, Reynolds CA, Danev R, Wootten D, Sexton PM

Published: ACS Pharmacol Transl Sci (2020)

Name: Nanobody 35

Organism: Lama glama

TM regions (from OPM database)

TM1(141-164),TM2(175-197),TM3(211-235),TM4(253-273),TM5(290-313),TM6(331-352),TM7(366-387)

Additional info

Membrane Thickness: 31 ± 1.4 Å

Tilt: 13 ± 4°