Rhodopsin-Gi protein complex

PDB ID: 6QNO RCSB | OPM

Receptor name: Rhodopsin

Receptor chain: R

Receptor organism: Bos taurus

Family / Class: Rhodopsin-like / A

Resolution [Å]: 4.4

Effector protein: G: α β γ (A B G)

Other protein: Fab antibody fragment light chain, Fab antibody fragment heavy chain

Non-peptide ligand code: RET (R)

MD preparation

Number of mutations: 3

List of mutations: (R)N2C (R)M257Y (R)D282C

Receptor

Name: Rhodopsin

Organism: Bos taurus

Uniprot: P02699

Name: Guanine nucleotide-binding protein G(i) subunit alpha-1

Organism: Homo sapiens

Uniprot: P63096

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Bos taurus

Uniprot: P62871

Name: Guanine nucleotide-binding protein G(T) subunit gamma-T1

Organism: Bos taurus

Uniprot: P02698

Title: Cryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the gβ subunit.

Authors: Tsai CJ, Marino J, Adaixo R, Pamula F, Muehle J, Maeda S, Flock T, Taylor NM, Mohammed I, Matile H, Dawson RJ, Deupi X, Stahlberg H, Schertler G

Published: Elife (2019)

Name: Fab antibody fragment light chain, Fab antibody fragment heavy chain

Organism: Mus musculus

TM regions (from OPM database)

TM1( 38-59),TM2( 75-99),TM3( 108-133),TM4( 155-173),TM5( 200-224),TM6( 253-274),TM7( 288-309)

Additional info

Membrane Thickness: 31 ± 1.8 Å

Tilt: 7 ± 1°