Cryo-EM structure of human cannabinoid receptor 2-Gi protein in complex with agonist WIN 55,212-2

PDB ID: 6PT0 RCSB | OPM

Receptor name: Cannabinoid receptor 2

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.2

Effector protein: G: α β γ (A B C)

Other protein: scFv16

Non-peptide ligand code: WI5 (R)

MD preparation

Number of mutations: 4

List of mutations: (A)S47N (A)G203A (A)E245A (A)A326S

Receptor

Name: Cannabinoid receptor 2

Organism: Homo sapiens

Uniprot: P34972

Name: Guanine nucleotide-binding protein G(i) subunit alpha-1

Organism: Homo sapiens

Uniprot: P63096

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Bos taurus

Uniprot: P62871

Name: Guanine nucleotide-binding protein G(T) subunit gamma-T1

Organism: Bos taurus

Uniprot: P02698

Title: Cryo-EM Structure of the Human Cannabinoid Receptor CB2-G_i Signaling Complex.

Authors: Xing C, Zhuang Y, Xu TH, Feng Z, Zhou XE, Chen M, Wang L, Meng X, Xue Y, Wang J, Liu H, McGuire TF, Zhao G, Melcher K, Zhang C, Xu HE, Xie XQ

Published: Cell (2020)

Name: scFv16

Organism: Synthetic construct

TM regions (from OPM database)

TM1( 34-56),TM2( 71-93),TM3( 107-129),TM4( 150-171),TM5( 189-211),TM6( 246-267),TM7( 280-301)

Additional info

Membrane Thickness: 30.2 ± 1 Å

Tilt: 3 ± 1°