Cryo-EM structure of Urocortin 1-bound Corticotropin-releasing factor 2 receptor in complex with Gs protein and Nb35

PDB ID: 6PB1 RCSB | OPM

Receptor name: Corticotropin-releasing factor receptor 2

Receptor chain: P

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 2.8

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

Peptide ligand: Urocortin (U)

MD preparation

Number of mutations: 2

List of mutations: (A)G226A (A)A366S

Receptor

Name: Corticotropin-releasing factor receptor 2

Organism: Homo sapiens

Uniprot: Q13324

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Molecular Basis for Hormone Recognition and Activation of Corticotropin-Releasing Factor Receptors.

Authors: Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE

Published: Mol Cell (2020)

Name: Nanobody 35

Organism: Synthetic construct

TM regions (from OPM database)

TM1( 114-139),TM2( 150-171),TM3( 183-210),TM4( 224-245),TM5( 264-288),TM6( 308-326),TM7( 335-362)

Additional info

Membrane Thickness: 31.4 ± 0.8 Å

Tilt: 4 ± 2°