Structure of human Smoothened-Gi complex

PDB ID: 6OT0 RCSB | OPM

Receptor name: Smoothened homolog

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Frizzled / F

Resolution [Å]: 3.8

Effector protein: G: α β γ (A B G)

Other protein: Fab light chain, Fab heavy chain

Non-peptide ligand code: CO1 (R)

MD preparation

Number of mutations: 0

List of mutations: None

Receptor

Name: Smoothened homolog

Organism: Homo sapiens

Uniprot: Q99835

Name: Guanine nucleotide-binding protein G(i) subunit alpha-1

Organism: Bos taurus

Uniprot: P04695

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Bos taurus

Uniprot: P62871

Name: Guanine nucleotide-binding protein G(T) subunit gamma-T1

Organism: Bos taurus

Uniprot: P02698

Title: Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric G_i.

Authors: Qi X, Liu H, Thompson B, McDonald J, Zhang C, Li X

Published: Nature (2019)

Name: Fab light chain, Fab heavy chain

Organism: Mus musculus

TM regions (from OPM database)

TM1( 230-253),TM2( 265-282),TM3( 315-337),TM4( 358-378),TM5( 399-420),TM6( 453-474),TM7( 516-537)

Additional info

Membrane Thickness: 31 ± 1 Å

Tilt: 4 ± 2°