Cryo-EM structure of formyl peptide receptor 2/lipoxin A4 receptor in complex with Gi

PDB ID: 6OMM RCSB | OPM

Receptor name: N-formyl peptide receptor 2

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.2

Effector protein: G: α β γ (A B C)

Other protein: scFv16

Peptide ligand: Peptide agonist (L)

Non-peptide ligand code: QXV (L)

MD preparation

Number of mutations: 8

List of mutations: (A)G203A (A)A326S (A)D328E (B)Q6E (B)E130Q (B)N237D (C)E17Q (C)E58Q

Receptor

Name: N-formyl peptide receptor 2

Organism: Homo sapiens

Uniprot: P25090

Name: Guanine nucleotide-binding protein G(i) subunit alpha-1

Organism: Homo sapiens

Uniprot: P63096

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Structure of formylpeptide receptor 2-G_i complex reveals insights into ligand recognition and signaling.

Authors: Zhuang Y, Liu H, Edward Zhou X, Kumar Verma R, de Waal PW, Jang W, Xu TH, Wang L, Meng X, Zhao G, Kang Y, Melcher K, Fan H, Lambert NA, Eric Xu H, Zhang C

Published: Nat Commun (2020)

Name: scFv16

Organism: Synthetic construct

TM regions (from OPM database)

TM1( 24-49),TM2( 65-86),TM3( 95-117),TM4( 146-163),TM5( 192-218),TM6( 244-269),TM7( 279-302)

Additional info

Membrane Thickness: 30.2 ± 1.4 Å

Tilt: 11 ± 0°