Cryo-EM structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and G protein

PDB ID: 6NBH RCSB | OPM

Receptor name: Parathyroid hormone/parathyroid hormone-related peptide receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 3.5

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

Peptide ligand: Long-acting parathyroid hormone analog (P)

MD preparation

Number of mutations: 1

List of mutations: (R)G188A

Receptor

Name: Parathyroid hormone/parathyroid hormone-related peptide receptor

Organism: Homo sapiens

Uniprot: Q03431

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short???

Organism: Bos taurus

Uniprot: P63097

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Rattus norvegicus

Uniprot: P54311

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Bos taurus

Uniprot: P63212

Title: Structure and dynamics of the active human parathyroid hormone receptor-1.

Authors: Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y

Published: Science (2019)

Name: Nanobody 35

Organism: Lama glama

TM regions (from OPM database)

TM1( 189-211),TM2( 220-241),TM3( 283-307),TM4( 321-342),TM5( 358-382),TM6( 406-426),TM7( 438-462)

Additional info

Membrane Thickness: 31.4 ± 0.8 Å

Tilt: 13 ± 1°