Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein

PDB ID: 6LPB RCSB | OPM

Receptor name: Pituitary adenylate cyclase-activating polypeptide type I receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 3.9

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

Peptide ligand: Pituitary adenylate cyclase-activating polypeptide (P)

MD preparation

Number of mutations: 0

List of mutations: None

Receptor

Name: Pituitary adenylate cyclase-activating polypeptide type I receptor

Organism: Homo sapiens

Uniprot: P41586

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Rattus norvegicus

Uniprot: P54311

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Bos taurus

Uniprot: P63212

Title: Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein.

Authors: Kobayashi K, Shihoya W, Nishizawa T, Kadji FMN, Aoki J, Inoue A, Nureki O

Published: Nat Struct Mol Biol (2020)

Name: Nanobody 35

Organism: Unidentified

TM regions (from OPM database)

TM1( 153-173),TM2( 187-209),TM3( 224-253),TM4( 267-287),TM5( 306-333),TM6( 346-369),TM7( 380-402)

Additional info

Membrane Thickness: 30.2 ± 0.6 Å

Tilt: 21 ± 0°