Cryo-EM structure of the human glucagon receptor in complex with Gi1

PDB ID: 6LML RCSB | OPM

Receptor name: Glucagon receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 3.9

Effector protein: G: α β γ (A B C)

Other protein: scFv16

Peptide ligand: Glucagon (E)

MD preparation

Number of mutations: 7

List of mutations: (A)S47N (A)G203A (A)E245A (A)A326S (R)E126R (R)T200W (R)A366M

Receptor

Name: Glucagon receptor

Organism: Homo sapiens

Uniprot: P47871

Name: Guanine nucleotide-binding protein G(i) subunit alpha-1

Organism: Homo sapiens

Uniprot: P63096

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Structural basis of G_s and G_i recognition by the human glucagon receptor.

Authors: Qiao A, Han S, Li X, Li Z, Zhao P, Dai A, Chang R, Tai L, Tan Q, Chu X, Ma L, Thorsen TS, Reedtz-Runge S, Yang D, Wang MW, Sexton PM, Wootten D, Sun F, Zhao Q, Wu B

Published: Science (2020)

Name: scFv16

Organism: Mus musculus

TM regions (from OPM database)

TM1( 140-161),TM2( 178-197),TM3( 221-246),TM4( 268-289),TM5( 300-323),TM6( 377-400)

Additional info

Membrane Thickness: 31.4 ± 0.8 Å

Tilt: 13 ± 0°