Cryo-EM structure of GPR52-miniGs-NB35

PDB ID: 6LI3 RCSB | OPM

Receptor name: G-protein coupled receptor 52

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.3

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

MD preparation

Number of mutations: 9

List of mutations: (R)A130W (R)C314P (A)G49D (A)E50N (A)A249D (A)S252D (A)I372A (A)V375I (G)C68S

Receptor

Name: G-protein coupled receptor 52

Organism: Homo sapiens

Uniprot: Q9Y2T5

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Structural basis of ligand recognition and self-activation of orphan GPR52.

Authors: Lin X, Li M, Wang N, Wu Y, Luo Z, Guo S, Han GW, Li S, Yue Y, Wei X, Xie X, Chen Y, Zhao S, Wu J, Lei M, Xu F

Published: Nature (2020)

Name: Nanobody 35

Organism: Mus musculus

TM regions (from OPM database)

TM1( 39-63),TM2( 78-102),TM3( 112-137),TM4( 158-178),TM5( 201-224),TM6( 266-290),TM7( 296-319)

Additional info

Membrane Thickness: 29.4 ± 2 Å

Tilt: 3 ± 0°