Cryo-EM structure of CB1-G protein complex

PDB ID: 6KPG RCSB | OPM

Receptor name: Cannabinoid receptor 1

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.0

Effector protein: G: α β γ (A B C)

Other protein: scFv16

Non-peptide ligand code: 8D0 (R)

MD preparation

Number of mutations: 3

List of mutations: (R)E273K (R)T283V (R)R340E

Receptor

Name: Cannabinoid receptor 1

Organism: Homo sapiens

Uniprot: P21554

Name: Guanine nucleotide-binding protein G(i) subunit alpha-1

Organism: Homo sapiens

Uniprot: P63096

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Activation and Signaling Mechanism Revealed by Cannabinoid Receptor-G_i Complex Structures.

Authors: Hua T, Li X, Wu L, Iliopoulos-Tsoutsouvas C, Wang Y, Wu M, Shen L, Johnston CA, Nikas SP, Song F, Song X, Yuan S, Sun Q, Wu Y, Jiang S, Grim TW, Benchama O, Stahl EL, Zvonok N, Zhao S, Bohn LM, Makriyannis A, Liu ZJ

Published: Cell (2020)

Name: scFv16

Organism: Homo sapiens

TM regions (from OPM database)

TM1( 118-142),TM2( 154-175),TM3( 190-212),TM4( 233-253),TM5( 273-295),TM6( 345-367),TM7( 378-400)

Additional info

Membrane Thickness: 30.2 ± 1.2 Å

Tilt: 4 ± 0°