3.3 angstrom phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor-Gs complex

PDB ID: 6B3J RCSB | OPM

Receptor name: Glucagon-like peptide 1 receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 3.3

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

Peptide ligand: Exendin-P5 (P)

MD preparation

Number of mutations: 8

List of mutations: (A)S54N (A)G226A (A)E268A (A)N271K (A)K274D (A)R280K (A)T284D (A)I285T

Receptor

Name: Glucagon-like peptide 1 receptor

Organism: Homo sapiens

Uniprot: P43220

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor-Gs complex.

Authors: Liang YL, Khoshouei M, Glukhova A, Furness SGB, Zhao P, Clydesdale L, Koole C, Truong TT, Thal DM, Lei S, Radjainia M, Danev R, Baumeister W, Wang MW, Miller LJ, Christopoulos A, Sexton PM, Wootten D

Published: Nature (2018)

Name: Nanobody 35

Organism: Lama glama

TM regions (from OPM database)

TM1( 143-167),TM2( 179-200),TM3( 224-249),TM4( 268-291),TM5( 303-325),TM6( 350-372),TM7( 379-405)

Additional info

Membrane Thickness: 31 ± 1.4 Å

Tilt: 15 ± 3°