Crystal structure of rhodopsin bound to visual arrestin determined by X-ray free electron laser

PDB ID: 5W0P RCSB | OPM

Receptor name: Rhodopsin

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.0

Effector protein: Arrestin (A)

Fusion protein: Endolysin

MD preparation

Number of mutations: 7

List of mutations: (A)N2C (A)E113Q (A)M257Y (A)N282C (A)L374A (A)V375A (A)F376A

The chain name of receptor was changed from A to R, Residue numbers of arrestin diminished by 2000

Receptor

Name: Rhodopsin

Organism: Homo sapiens

Uniprot: P08100

Fusion protein

Name: Endolysin

Organism: Enterobacteria phage RB59

Uniprot: P00720

Name: S-arrestin

Organism: Mus musculus

Uniprot: P20443

Title: Identification of Phosphorylation Codes for Arrestin Recruitment by G Protein-Coupled Receptors.

Authors: Zhou XE, He Y, de Waal PW, Gao X, Kang Y, Van Eps N, Yin Y, Pal K, Goswami D, White TA, Barty A, Latorraca NR, Chapman HN, Hubbell WL, Dror RO, Stevens RC, Cherezov V, Gurevich VV, Griffin PR, Ernst OP, Melcher K, Xu HE

Published: Cell (2017)

TM regions (from OPM database)

TM1( 38-63),TM2( 73-99),TM3( 109-135),TM4( 151-173),TM5( 202-225),TM6( 253-274),TM7( 286-309)

Additional info

Membrane Thickness: 31.8 ± 1 Å

Tilt: 8 ± 0°