Volta phase plate cryo-electron microscopy structure of a calcitonin receptor-heterotrimeric Gs protein complex

PDB ID: 5UZ7 RCSB | OPM

Receptor name: Calcitonin receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 4.1

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

MD preparation

Number of mutations: 0

List of mutations: None

Receptor

Name: Calcitonin receptor

Organism: Homo sapiens

Uniprot: P30988

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768

Title: Phase-plate cryo-EM structure of a class B GPCR-G-protein complex.

Authors: Liang YL, Khoshouei M, Radjainia M, Zhang Y, Glukhova A, Tarrasch J, Thal DM, Furness SGB, Christopoulos G, Coudrat T, Danev R, Baumeister W, Miller LJ, Christopoulos A, Kobilka BK, Wootten D, Skiniotis G, Sexton PM

Published: Nature (2017)

Name: Nanobody 35

Organism: Lama glama

TM regions (from OPM database)

TM1( 147-172),TM2( 182-203),TM3( 221-242),TM4( 259-281),TM5( 298-317),TM6( 346-360),TM7( 370-393)

Additional info

Membrane Thickness: 30.2 ± 1 Å

Tilt: 13 ± 0°