Crystal structure of rhodopsin bound to visual arrestin

PDB ID: 5DGY RCSB | OPM

Receptor name: Rhodopsin

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 7.7

Effector protein: Arrestin (A)

Fusion protein: Endolysin

MD preparation

Number of mutations: 7

List of mutations: (R)N2C (R)E113Q (R)M257Y (R)N282C (A)L374A (A)V375A (A)F376A

The chain name of receptor was changed from A to R, Residue numbers of arrestin diminished by 1000

Receptor

Name: Rhodopsin

Organism: Homo sapiens

Uniprot: P08100

Fusion protein

Name: Endolysin

Organism: Enterobacteria phage RB59

Uniprot: P00720

Name: S-arrestin

Organism: Mus musculus

Uniprot: P20443

Title: X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex.

Authors: Zhou XE, Gao X, Barty A, Kang Y, He Y, Liu W, Ishchenko A, White TA, Yefanov O, Han GW, Xu Q, de Waal PW, Suino-Powell KM, Boutet S, Williams GJ, Wang M, Li D, Caffrey M, Chapman HN, Spence JC, Fromme P, Weierstall U, Stevens RC, Cherezov V, Melcher K, Xu HE

Published: Sci Data (2016)

TM regions (from OPM database)

TM1( 35-63),TM2( 73-99),TM3( 109-134),TM4( 151-173),TM5( 201-224),6( 253-277),TM7( 286-309)

Additional info

Membrane Thickness: 31.8 ± 1.1 Å

Tilt: 9 ± 1°