Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

PDB ID: 4ZWJ RCSB | OPM

Receptor name: Rhodopsin

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.3

Effector protein: Arrestin (A)

Fusion protein: Endolysin

MD preparation

Number of mutations: 7

List of mutations: (R)N2C (R)E113Q (R)M257Y (R)N82C (R)L374A (R)V375A (R)F376A

The chain name of receptor was changed from A to R

Receptor

Name: Rhodopsin

Organism: Homo sapiens

Uniprot: P08100

Fusion protein

Name: Endolysin

Organism: Enterobacteria phage RB59

Uniprot: P00720

Name: S-arrestin

Organism: Mus musculus

Uniprot: P20443

Title: Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser.

Authors: Kang Y, Zhou XE, Gao X, He Y, Liu W, Ishchenko A, Barty A, White TA, Yefanov O, Han GW, Xu Q, de Waal PW, Ke J, Tan MH, Zhang C, Moeller A, West GM, Pascal BD, Van Eps N, Caro LN, Vishnivetskiy SA, Lee RJ, Suino-Powell KM, Gu X, Pal K, Ma J, Zhi X, Boutet S, Williams GJ, Messerschmidt M, Gati C, Zatsepin NA, Wang D, James D, Basu S, Roy-Chowdhury S, Conrad CE, Coe J, Liu H, Lisova S, Kupitz C, Grotjohann I, Fromme R, Jiang Y, Tan M, Yang H, Li J, Wang M, Zheng Z, Li D, Howe N, Zhao Y, Standfuss J, Diederichs K, Dong Y, Potter CS, Carragher B, Caffrey M, Jiang H, Chapman HN, Spence JC, Fromme P, Weierstall U, Ernst OP, Katritch V, Gurevich VV, Griffin PR, Hubbell WL, Stevens RC, Cherezov V, Melcher K, Xu HE

Published: Nature (2015)

TM regions (from OPM database)

TM1( 35-63),TM2( 73-99),TM3( 109-134),TM4( 151-173),TM5( 201-224),6( 253-277),TM7( 286-309)

Additional info

Membrane Thickness: 31.8 ± 1.1 Å

Tilt: 9 ± 1°