General description

Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein


Receptor name: Prostaglandin E2 receptor EP4 subtype

Receptor chain: A

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.3

Effector protein: G: α β γ (D B C)

Other protein: nanobody Nb35 (E)

Non-peptide ligand code: P2E (A)

MD preparation


Number of mutations: 9

List of mutations: (A)N7Q (A)N177Q (D)G49D (D)E50N (D)L63Y (D)A249D (D)S252D (D)I362A (D)V365I


Name: Prostaglandin E2 receptor EP4 subtype

Organism: Homo sapiens

Uniprot: P35408

Effector protein

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768


Title: Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein.

Authors: Nojima, S., Fujita, Y., Kimura, K.T., Nomura, N., Suno, R., Morimoto, K., Yamamoto, M., Noda, T., Iwata, S., Shigematsu, H., Kobayashi, T.

Published: Structure (2020)

Other protein (not shown in structure)

Name: nanobody Nb35 (E)

Organism: Lama glama

Receptor TM helicies
TM regions (from OPM database)

TM1( 21-43),TM2( 56-78),TM3( 90-118),TM4( 136-156),TM5( 180-208),TM6( 227-251),TM7( 270-291)

Additional info

Membrane Thickness: 31.4 ± 1.0 Å

Tilt: 19 ± 1°

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