General description

Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex


Receptor name: Growth hormone-releasing hormone receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 2.6

Effector protein: G: α β γ (A B G)

Other protein: Nanobody35 (N)

Peptide ligand: Somatoliberin (P)

MD preparation


Number of mutations: 8

List of mutations: (A)S54N, (A)G226A, (A)E268A, (A)N271K, (A)K274D, (A)R280K, (A)T284D, (A)I285T


Name: Growth hormone-releasing hormone receptor

Organism: Homo sapiens

Uniprot: Q02643

Effector protein

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Rattus norvegicus

Uniprot: P54311

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Bos taurus

Uniprot: P63212


Title: Structural basis for activation of the growth hormone-releasing hormone receptor.

Authors: Zhou, F., Zhang, H., Cong, Z., Zhao, L.H., Zhou, Q., Mao, C., Cheng, X., Shen, D.D., Cai, X., Ma, C., Wang, Y., Dai, A., Zhou, Y., Sun, W., Zhao, F., Zhao, S., Jiang, H., Jiang, Y., Yang, D., Eric Xu, H., Zhang, Y., Wang, M.W.

Published: Nat Commun (2020)

Other protein (not shown in structure)

Name: Nanobody35 (N)

Organism: synthetic construct

Receptor TM helicies
TM regions (from OPM database)

TM1( 128-153),TM2( 161-185),TM3( 200-229),TM4( 241-266),TM5( 281-305),TM6( 324-347),TM7( 353-380)

Additional info

Membrane Thickness: 35.8 ± 3.0 Å

Tilt: 11 ± 1°

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