General description

Cryo-EM structure of human cannabinoid receptor 2-Gi protein in complex with agonist WIN 55,212-2


Receptor name: Cannabinoid receptor 2

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.2

Effector protein: G: α β γ (A B C)

Other protein: scFv16

Non-peptide ligand code: WI5 (R)

MD preparation


Number of mutations: 4

List of mutations: (A)S47N (A)G203A (A)E245A (A)A326S


Name: Cannabinoid receptor 2

Organism: Homo sapiens

Uniprot: P34972

Effector protein

Name: Guanine nucleotide-binding protein G(i) subunit alpha-1

Organism: Homo sapiens

Uniprot: P63096

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Bos taurus

Uniprot: P62871

Name: Guanine nucleotide-binding protein G(T) subunit gamma-T1

Organism: Bos taurus

Uniprot: P02698


Title: Cryo-EM Structure of the Human Cannabinoid Receptor CB2-G<sub>i</sub> Signaling Complex.

Authors: Xing C, Zhuang Y, Xu TH, Feng Z, Zhou XE, Chen M, Wang L, Meng X, Xue Y, Wang J, Liu H, McGuire TF, Zhao G, Melcher K, Zhang C, Xu HE, Xie XQ

Published: Cell (2020)

Other protein (not shown in structure)

Name: scFv16

Organism: Synthetic construct

Receptor TM helicies
TM regions (from OPM database)

TM1( 34-56),TM2( 71-93),TM3( 107-129),TM4( 150-171),TM5( 189-211),TM6( 246-267),TM7( 280-301)

Additional info

Membrane Thickness: 30.2 ± 1 Å

Tilt: 3 ± 1°

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