General description

Cryo-EM structure of GPR52-miniGs-NB35


Receptor name: G-protein coupled receptor 52

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 3.3

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

MD preparation


Number of mutations: 9

List of mutations: (R)A130W (R)C314P (A)G49D (A)E50N (A)A249D (A)S252D (A)I372A (A)V375I (G)C68S


Name: G-protein coupled receptor 52

Organism: Homo sapiens

Uniprot: Q9Y2T5

Effector protein

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768


Title: Structural basis of ligand recognition and self-activation of orphan GPR52.

Authors: Lin X, Li M, Wang N, Wu Y, Luo Z, Guo S, Han GW, Li S, Yue Y, Wei X, Xie X, Chen Y, Zhao S, Wu J, Lei M, Xu F

Published: Nature (2020)

Other protein (not shown in structure)

Name: Nanobody 35

Organism: Mus musculus

Receptor TM helicies
TM regions (from OPM database)

TM1( 39-63),TM2( 78-102),TM3( 112-137),TM4( 158-178),TM5( 201-224),TM6( 266-290),TM7( 296-319)

Additional info

Membrane Thickness: 29.4 ± 2 Å

Tilt: 3 ± 0°

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