General description

Cryo-EM structure of the adenosine A2A receptor bound to a miniGs heterotrimer


Receptor name: Adenosine receptor A2a

Receptor chain: A

Receptor organism: Homo sapiens

Family / Class: Rhodopsin-like / A

Resolution [Å]: 4.1

Effector protein: G: α β γ (D B C)

Fusion protein: Thioredoxin 1

Other protein: Nanobody 35

Non-peptide ligand code: NEC (A)

MD preparation


Number of mutations: 0

List of mutations: None


Name: Adenosine receptor A2a

Organism: Homo sapiens

Uniprot: P29274

Fusion protein

Name: Thioredoxin 1

Organism: Escherichia coli

Uniprot: P0AA25

Effector protein

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Mus musculus

Uniprot: P62874

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768


Title: Cryo-EM structure of the adenosine A<sub>2A</sub> receptor coupled to an engineered heterotrimeric G protein.

Authors: García-Nafría J, Lee Y, Bai X, Carpenter B, Tate CG

Published: Elife (2018)

Other protein (not shown in structure)

Name: Nanobody 35

Organism: Lama glama

Receptor TM helicies
TM regions (from OPM database)

TM1( 9-28),TM2( 43-68),TM3( 76-103),TM4( 119-141),TM5( 174-200),TM6( 232-255),TM7( 267-288)

Additional info

Membrane Thickness: 32.2 ± 1.6 Å

Tilt: 14 ± 4°

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