General description

3.3 angstrom phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor-Gs complex


Receptor name: Glucagon-like peptide 1 receptor

Receptor chain: R

Receptor organism: Homo sapiens

Family / Class: Secretin / B1

Resolution [Å]: 3.3

Effector protein: G: α β γ (A B G)

Other protein: Nanobody 35

Peptide ligand: Exendin-P5 (P)

MD preparation


Number of mutations: 8

List of mutations: (A)S54N (A)G226A (A)E268A (A)N271K (A)K274D (A)R280K (A)T284D (A)I285T


Name: Glucagon-like peptide 1 receptor

Organism: Homo sapiens

Uniprot: P43220

Effector protein

Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short

Organism: Homo sapiens

Uniprot: P63092

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1

Organism: Homo sapiens

Uniprot: P62873

Name: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2

Organism: Homo sapiens

Uniprot: P59768


Title: Phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor-Gs complex.

Authors: Liang YL, Khoshouei M, Glukhova A, Furness SGB, Zhao P, Clydesdale L, Koole C, Truong TT, Thal DM, Lei S, Radjainia M, Danev R, Baumeister W, Wang MW, Miller LJ, Christopoulos A, Sexton PM, Wootten D

Published: Nature (2018)

Other protein (not shown in structure)

Name: Nanobody 35

Organism: Lama glama

Receptor TM helicies
TM regions (from OPM database)

TM1( 143-167),TM2( 179-200),TM3( 224-249),TM4( 268-291),TM5( 303-325),TM6( 350-372),TM7( 379-405)

Additional info

Membrane Thickness: 31 ± 1.4 Å

Tilt: 15 ± 3°

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